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Intracellular adaptation of Brucella abortus
(Universidad Nacional, Costa Rica, 2009-03)
Macrophages were infected with virulent B. abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared. The virulent strain initially ...
Activation of Rho and Rab GTPases dissociates Brucella abortus internalization from intracellular trafficking
(Blackwell Science, 2002-10-04)
Brucella abortus is an intracellular pathogen that relies on unconventional virulence factors to infect hosts. In non-professional phagocytes, Rho GTPasesactivation by the Escherichia coli cytotoxic necrotiz ing factor ...
GTPases of the Rho subfamily are required for Brucella abortus internalization in nonprofessional phagocytes
(The American Society for Biochemistry and Molecular Biology, Inc., 2001-11-30)
Members of the genus Brucella are intracellular -Proteobacteria
responsible for brucellosis, a chronic disease
of humans and animals. Little is known about Brucella
virulence mechanisms, but the abilities of these ...
In vivo proteolytic degradation of the Escherichia coli acyltransferase HlyC
(The American Society for Biochemistry and Molecular Biology, 2001-05-18)
Escherichia coli hemolysin (HlyA) is the prototype toxin of a major family of exoproteins produced by Gram-negative bacteria known as “repeats in toxins.” Only fatty acid-acylated HlyA molecules at residues Lys564 and ...
Purification of intracellular bacteria: isolation of viable Brucella abortus from host cells
(Springer Science, Business Media Nueva York, 2014, 2014)
The pathogenesis of brucellosis depends on the ability of bacteria from the genus Brucella to invade and
replicate within animal cells. To understand the molecular pathways used by Brucella spp. to reach its
intracellular ...
Bacterial counts in spleen
(Bio-protocol, 2013-11-05)
Bacterial loads can be determined as colony forming units (CFU) at any point of the infection by culturing spleen homogenates on agar plates. This is a reliable method for comparing the kinetics of infection in various ...
Brucella abortus ornithine lipids are dispensable outer membrane components devoid of a marked pathogen-associated molecular pattern
(PLoS One, 2011-01-07)
The brucellae are a-Proteobacteria facultative intracellular parasites that cause an important zoonosis. These bacteria escape early detection by innate immunity, an ability associated to the absence of marked pathogen-associated ...
Estandarización de un protocolo de caracterización molecular para la identificación de especie de cepas terrestres de bacterias del género Brucella
(Ediciones Unisalle, 2011-10-03)
La brucelosis es la enfermedad zoonótica más extendida en el mundo que afecta diversos animales, incluyendo especies domésticas y de vida silvestre. Es causada por una
bacteria perteneciente al género Brucella, el cual ...
Platelet depletion does not alter the course of Brucella abortus infection in vivo
(Elsevier, 2022-02-23)
Brucellosis is a bacterial disease of animals and a zoonotic infection. Thrombocytopenia is a common outcome in
long-lasting brucellosis in humans. Likewise, ex vivo experiments have shown that platelets may play a role ...
Antimicrobial activity of myotoxic phospholipases A2 from crotalid snake venoms and synthetic peptide variants derived from their C-terminal region
(Elsevier, 2005)
A short peptide derived from the C-terminal region of Bothrops asper myotoxin II, a Lys49 phospholipase A2 (PLA2), was previously found to reproduce the bactericidal activity of its parent molecule. In this study, a panel ...