Comparison of the serum neutralization test and a competitive enzyme-linked immunosorbent assay for the detection of antibodies to vesicular stomatitis virus New Jersey and vesicular stomatitis virus Indiana
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Date
2002-05-01Author
Alvarado, Juan Francisco
Herrero, Marco V.
McCluskey, Brian
Salman, Mo
Dolz, Gaby
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A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against
vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with
the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms
in the Poa´s region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95%
confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests,
respectively. These results indicate good to excellent agreement between the 2 tests under these conditions. Ensayo inmunoabsorbente ligado a enzimas competitivo (C-ELISA) para la detección de anticuerpos contra
El virus de la estomatitis vesicular de Nueva Jersey (VSV-NJ) y el virus de la estomatitis vesicular de Indiana (VSV-IN) se compararon con
la prueba de seroneutralización (SNT) utilizando 1106 muestras de suero obtenidas de ganado lechero en granjas de estudio centinela
en la región del Poa de Costa Rica. Los coeficientes Kappa entre el C-ELISA y el SNT fueron 0,8871 (95%
intervalo de confianza [IC]: 0,8587–0,9155) y 0,6912 (IC 95 %: 0,6246–0,7577) para las pruebas VSV-NJ y VSV-IN,
respectivamente. Estos resultados indican una concordancia de buena a excelente entre las 2 pruebas en estas condiciones.
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